Human gp130 Alexa Fluor 700 MAb (Clone 28126) (100 TESTS)
货号：
FAB228N-100 基本售价：
4808.5 元 规格：
100 Tests
产品信息
概述| 货号 | FAB228N-100 |
| 别名 | CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; GP130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha |
全称 | Glycoprotein 130 | | 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells)
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| 目标/特异性 | Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3572 (Human); 16195 (Mouse); 25205 (Rat) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2-5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Wang, Y. et al. (1992) Genomics 14:666.
- Muller-Newen, G. (2003) Sci. STKE pe40.
- Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
- Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
- Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
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参考图片Detection of gp130 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human gp130 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB228N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins. |
