概述| 货号 | IC1835N |
| 别名 | CED; DPD1; LAP; TGFB; TGFB1; TGFbeta; transforming growth factor, beta 1 |
全称 | Transforming Growth Factor beta 1, 2, and 3 | | 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells)
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| 目标/特异性 | Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Bovine bone-derived TGF-beta 1 and TGF-beta 2 |
| 标记 | Alexa Fluor 700 |
| 背景 | TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Ayala A. et al. (1992) FASEB J. 6:A1604.
- Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
- Dasch J.R. et al. (1989) J. Immunol. 142:1536.
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参考图片Detection of TGF-beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # IC1835N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
