货号 | IC1259A |
别名 | FLJ21841; NES; nestin |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects human Nestin in flow cytometry. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 10763 (Human); 18008 (Mouse); 25491 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chemokine receptor CXCR3 promotes growth of glioma. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | NS0 mouse myeloma cell line transfected with human Nestin Accession # P48681 |
生物活性 | Human |
标记 | Allophycocyanin |
背景 | Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in both the neural differentiation pathway and that of CNS glia. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Full-length human Nestin shares 51% amino acid sequence identity to mouse Nestin. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin APC‑conjugated Monoclonal Antibody (Catalog # IC1259A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Intracellular Molecules. |