Human IL-18 R alpha/IL-1 R5 Allophycocyanin MAb (70625) (100 TESTS)【科瑞奥博】

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Human IL-18 R alpha/IL-1 R5 Allophycocyanin MAb (70625) (100 TESTS)

货号： FAB840A-100 基本售价： 4808.5 元规格： 100 Tests

产品信息

概述
货号	FAB840A-100
别名	CD218a; IL-1 R5; IL-1 Rrp; IL-18 R1; IL18R1
全称	Interleukin 18 Receptor alpha
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8809 (Human); 16174 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells Authors: Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He Mediators Inflamm., 2017;2017(0):3090782. Species: Human Sample Type: Whole Cells Application: Flow Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He J. Cell. Mol. Med., 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5 Glu20-Arg329 Accession # Q13478
生物活性	Human
标记	Allophycocyanin
背景	Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Parnet, P.et al. (1996) J. Biol. Chem. 271:3967. Torigoe, K.et al. (1997) J. Biol. Chem. 272:25737. Born, T.L.et al. (1998) J. Biol. Chem. 273:29445.

参考图片

Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA (5 μg/ml) and Recombinant Human IL‑2 (Catalog # 202-IL;10 ng/ml) for 2 days were stained with Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Monoclonal Antibody (Catalog # FAB840A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.