货号 | IC1420N |
别名 | AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin | 全称 | Actin, Alpha 2, Smooth Muscle, Aorta |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects human alpha -Smooth Muscle Actin. |
使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 59 (Human); 11475 (Mouse); 81633 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Deciphering the stromal and hematopoietic cell network of the adventitia from non-aneurysmal and aneurysmal human aorta. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide |
生物活性 | Human |
标记 | Alexa Fluor 700 |
背景 | alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of alpha ‑Smooth Muscle Actin in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1420N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |