Human FAP Alexa Fluor 700 MAb (Clone 427819) (100 UG)【科瑞奥博】

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Human FAP Alexa Fluor 700 MAb (Clone 427819) (100 UG)

货号： FAB3715N-100UG 基本售价： 4659.8 元规格： 100 UG

产品信息

概述
货号	FAB3715N-100UG
别名	170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin
应用	Flow Cytometry
目标/特异性	Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 is observed.
使用方法	Flow Cytometry: 0.25-1 µg/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2191 (Human); 14089 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP Leu26-Asp760 Accession # Q12884
生物活性	Human
标记	Alexa Fluor 700
背景	FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin alpha ₃beta₁, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.
运输条件	Blue Ice
存放说明	4℃
参考文献	Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595. OBrien, P. and B.F. OConnor (2008) Biochim. Biophys. Acta 1784:1130. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

参考图片

Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. WI‑38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein alpha /FAP Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3715N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.