Human CXCL4/PF4 Alexa Fluor 350 Antibody (Clone 170138) (100 UG)
货号：
IC7952U-100UG 基本售价：
4659.8 元 规格：
100 UG
产品信息
概述| 货号 | IC7952U-100UG |
| 别名 | chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human CXCL4/PF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) CXCL6, recombinant human (rh) CXCL6, rhCXCL7, rmCXCL13 or rhCCL21 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5196 (Human); 56744 (Mouse); 360918 (Rat) |
| 免疫原 | E. coli-derived recombinant human CXCL4/PF4
Glu32-Ser101
Accession # P02776.2 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 350 |
| 背景 | CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and
heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in
alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Poncz, M. et al. (1987) Blood 69:219.
- Scheuerer, B. et al. (2000) Blood 95:1158.
- Perollet, C. et al. (1998) Blood 91:3289.
- Petersen, F. et al. (1998) J. Immunol. 161:4347.
- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
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