| 货号 | FAB58891N-100UG |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Klotho beta in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 152831 (Human); 83379 (Mouse); 289625 (Rat) |
| 免疫原 | Mouse myeloma cell line, NS0-derived recombinant human Klotho beta with a C-terminal 10 His tag. Phe53-Leu997 Accession # Q86Z14 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1, 2). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (3). The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-5). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2-4, 6). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 7). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (8). The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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