| 货号 | FAB4894R-100UG |
| 别名 | CD312; DKFZp781B135; egf-like module containing, mucin-like, hormone receptor-like 2; egf-like module containing, mucin-like, hormone receptor-like sequence 2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; EMR2 | 全称 | EGF-like Module Containing, Mucin-like, Hormone Receptor-like 2 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human EMR2 in EMR2-transfected cells but not in the parental cell line. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 30817 (Human) |
| 免疫原 | NS0 mouse myeloma cell line transfected with human EMR2 Gln24-Asn823 Accession # AAI27006 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 647 |
| 背景 | EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479‑530) and a 7‑TM sequence (aa 531‑785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1‑478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2‑5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4‑6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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