| 货号 | IC6456T-100UG |
| 别名 | cytokine receptor; EBI3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL-27/IL-35 EBI3 Subunit; IL27B; IL-27B; IL35 EBI3; interleukin-27 subunit beta | 全称 | Epstein-Barr virus induced gene 3 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit Arg21-Lys229 (predicted) Accession # Q14213 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 594 |
| 背景 | IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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