| 货号 | FAB676S-100UG |
| 别名 | C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3; FLJ37788; IL-1 R3; IL-1 RAcP; IL-1 receptor accessory protein; IL-1R3; IL-1R-3; IL1RAcP; IL-1RAcP; IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin-1 receptor accessory protein | 全称 | Interleukin 1 Receptor Accessory Protein |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human IL-1RAcP/IL-1 R3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhST2, rhIL-18 R alpha, IL-1 Rrp2, rhIL-18 RAP, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhTLR1, rhTLR2, rhTLR3, rhTLR4, rhTLR5, rhTLR7, rhTLR8, rhTLR9, rhTLR10, rhMD-1, rhMD-2, or recombinant mouse RP105 is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3556 (Human); 16180 (Mouse) |
| 免疫原 | S. frugiperda insect ovarian cell line Sf21-derived recombinant human IL-1RAcP/IL-1 R3 Ser21-Glu359 (predicted) Accession # Q9NPH3 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 750 |
| 背景 | IL-1 Receptor Accessory Protein (also IL-1 R3) is a ubiquitous 70-90 kDa member of the interleukin-1 receptor family of proteins (1-5). It serves as a non-ligand-binding accessory component of the receptors for IL-1 alpha, IL-1 beta, and IL-33 (6, 7). Together with IRAK4 and MyD88, it generates a functional signaling complex with IL-1 RI; by itself, it generates a non-signaling, but high-affinity binding complex with IL-1 RII (8). In addition, it interacts with ST2 on mast cells and Th2 T cells to create a functional IL-33 receptor complex (7). Mature human IL-1 RAcP is a type I transmembrane glycoprotein that is 550 amino acids in length. It contains a 347 amino acid (aa) extracellular region (aa 21-367), a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (9). The extracellular region shows three C2-type Ig-like domains, the most membrane proximal of which is suggested to be responsible for dimerization with IL-1 RI (10). There are three alternative splice forms reported for IL-1 RAcP. One is transmembrane and shows a 239 aa substitution for the C-terminal 122 amino acids (11). The other two are soluble; one shows a six aa substitution for aa 351-570, while a second shows a 45 aa substitution for aa 302-579 (12, 13). The soluble receptor isoforms appear to be inhibitory to IL-1 signaling. When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (14). The human and mouse IL-1 RAcP precursors are 89% aa identical; within the extracellular region, they share 86% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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