Human Vimentin Alexa Fluor 488 Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Human Vimentin Alexa Fluor 488 Affinity Purified PAb (100 TESTS)

货号： IC8104G 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	IC8104G
别名	FLJ36605; VIM; vimentin
反应种属	Human
应用	Immunocytochemistry(1:10 dilution)
目标/特异性	Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. The unconjugated antibody detects human Vimentin in Western blots.
使用方法	Immunocytochemistry: 1:10 dilution
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7431 (Human); 22352 (Mouse); 81818 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Vimentin Ser2-Glu466 Accession # P08670
生物活性	Human
标记	Alexa Fluor 488
背景	Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919. Li, Q.-F. et al. (2006) J. Biol. Chem. 281:34716. Esue, O. et al. (2006) J. Biol. Chem. 281:30393. Styers, M.L. et al. (2005) Traffic 6:359. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156. Ivaska, J. et al. (2005) EMBO J. 24:3834. Byun, Y. et al. (2001) Cell Death Differ. 8:443. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625. Garg, A. et al. (2006) J. Immunol. 177:6192.

参考图片

Vimentin in HeLa Human Cell Line. Vimentin was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Vimentin Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC8104G) at 1:10 dilution overnight at 4 ° C and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.