| 货号 | IC7986G |
| 别名 | Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4Hbeta; p55; PDI; PDIA1; PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1); PDIEC 5.3.4.1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide; PROHB; PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide-isomerase; protocollagen hydroxylase; Thbp; thyroid hormone-binding protein p55 | 全称 | Proline 4-Hydroxylase, Beta Polypeptide |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(1:10 dilution) |
| 目标/特异性 | Designed to visualize the expression of P4HB by fluorescence microscopy for staining cells and tissues. Conjugated P4HB antibodies are ideal for immunocytochemistry colocalization studies in the lumen of the endoplasmic reticulum. The unconjugated antibody detects human P4HB in direct ELISAs and Western blots. |
| 使用方法 | Immunocytochemistry: 1:10 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5034 (Human); 18453 (Mouse); 25506 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human P4HB Asp18-Lys505 Accession # P07237 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family. As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4 hydroxylase. This catalyses the hydroxylation of proline in collagen. On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells. Mature human P4HB is 491 amino acids (aa) in length. It contains two TRX domains (aa 25 134 and 368 475) plus an ER retention sequence (aa 505 508). There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18 505, human P4HB shares 94% aa identity with mouse P4HB. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Protein Disulfide Isomerase/P4HB in HeLa Human Cell Line. Protein Disulfide Isomerase/P4HB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Protein Disulfide Isomerase/P4HB Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC7986G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to the endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
