| 货号 | IC5664G |
| 别名 | STX6; syntaxin 6; syntaxin-6 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(1:10 dilution) |
| 目标/特异性 | Conjugated Syntaxin 6 antibodies are ideal for immunocytochemistry colocalization studies in trans-Golgi and endosomal membranes. The unconjugated antibody detects human Syntaxin 6 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross‑reactivity with recombinant human (rh) Syntaxin 8, rhSyntaxin 12, and rhSyntaxin 16 is observed. |
| 使用方法 | Immunocytochemistry: 1:10 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10228 (Human); 58244 (Mouse); 60562 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin 6 Ser69-Asn217 Accession # O43752 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Syntaxin 6 in HeLa Human Cell Line. Syntaxin 6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5664G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to Golgi bodies. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
