货号 | IC2400G |
别名 | forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B | 全称 | Hepatocyte Nuclear Factor-3, beta |
反应种属 | Human |
应用 | Immunocytochemistry(10 µg/mL) Intracellular Staining by Flow Cytometry(5 µL/106cells) |
目标/特异性 | Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots. |
使用方法 | Immunocytochemistry: 10 µg/mL Intracellular Staining by Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3170 (Human) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human HNF‑3 beta /FoxA2 Met242-Ser457 Accession # Q9Y261 |
生物活性 | Human |
标记 | Alexa Fluor 488 |
背景 | HNF-3 beta, also known as FoxA2, is a member of the forkhead class ofDNA-binding proteins. It is a transcriptional activator forliver-specific transcripts such as albumin and transthyretin. Similarfamily members play roles in the differentiation of the pancreas andliver. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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HNF-3 beta /Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells. BG01V human embryonic stem cells were differentiated into mesendoderm using the StemXVivo Endoderm Kit (Catalog # SC019). HNF-3 beta /FoxA2 was detected in immersion fixed mesendoderm differentiated BG01V human embryonic stem cells using 10 μg/mL Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, green) for 3 hours at room temperature and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of HNF‑3 beta /FoxA2 in Human iPS Cells by Flow Cytometry. Human iPS cells were stained with Goat Anti-Human HNF‑3 beta /FoxA2 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |