Human Phospho-Axl (Y779) Alexa Fluor 488 MAb (Clone 713610) (100 TESTS)
货号：
IC6965G 基本售价：
4659.8 元 规格：
100 Tests
产品信息
概述| 货号 | IC6965G |
| 别名 | Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL |
全称 | Axl Receptor Tyrosine Kinase | | 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells)
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| 目标/特异性 | Detects human Phospho-Axl (Y779) in direct ELISAs and Western blots.
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| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 558 (Human); 26362 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Phosphopeptide containing the human Axl Y779 site |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Phosphorylation of Tyrosine 779 provides a docking site for p85 subunits of PI 3-Kinase (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
- Weinger, J.G. et al. (2008) J. Neurochem. 106:134.
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参考图片Detection of Phospho-Axl (Y779) in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line (A) resting or (B) treated with 100 nM Pervanadate for 10 minutes was stained with Mouse Anti-Human Phospho-Axl (Y779) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6965G) and Mouse Anti-Human Axl APC‑conjugated Monoclonal Antibody (Catalog # FAB154A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
