Human LAP (TGF-beta 1) Alexa Fluor 700 MAb (Clone 27232) (100 TESTS)【科瑞奥博】

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Human LAP (TGF-beta 1) Alexa Fluor 700 MAb (Clone 27232) (100 TESTS)

货号： FAB2463N 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	FAB2463N
别名	LAP (TGFbeta 1); LAP (TGF-beta 1)
全称	Latency-associated Peptide
反应种属	Human
应用	Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha.
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7040 (Human)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1) Leu30-Arg278 (Cys33Ser) Accession # P01137
生物活性	Human
标记	Alexa Fluor 700
背景	TGF-beta 1 is one member of a six gene family that has been described in mammals, birds, fish and frog. The name TGF-beta is applied to a 24-28 kDa disulfide-linked dimer that is generated through the proteolytic processing of a larger precursor molecule. For TGF-beta 1, a 50-55 kDa, 391 amino acid (aa) proprecursor is first, covalently linked to a second proprecursor (creating a disulfide-linked homodimer), and second, internally cleaved to generate two covalently-linked homodimers that remain non-covalently associated. The smallest homodimer representing aa 279-390 of the proform is TGF-beta 1; the largest homodimer representing aa 30-278 of the proform is termed LAP (Latency-associated Peptide). The LAP homodimer wraps itself around the smaller TGF-beta 1 homodimer, thus blocking an interaction of mature TGF-beta with its receptors. Almost all cells secrete the inactive TGF-beta :LAP complex, and most do so with LAP covalently bound to a very large 120-160 kDa LTBP (Latent TGF-beta Binding Protein), platelets being a notable exception. LTBP associates with multiple matrix components and this serves to store TGF-beta extracellularly in a non-active form. When TGF-beta signaling is needed, the LTBP:matrix association is disrupted, and the TGF-beta :LAP complex is exposed to multiple LAP binding partners such as TSP-1 and various Integrins. Interactions with these factors cause LAP to unwrap and dissociate from TGF-beta, resulting in TGF-beta "activation" and receptor binding. Ther are three human TGF-beta 1 LAPs and TGF-beta 1 LAP shares 36% and 33% aa sequence indentity with TGF-beta 2 LAP and TGF-beta 3 LAP, respectively. Human to mouse, TGF-beta 1 LAP shares 86% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cytokines and Receptors in Angiogenesis gamma delta T Cells Interactive Pathway: TGF-beta Signaling Pathways Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) T Cell Cytokine Signaling TGF-beta Family TGF-beta Family Ligands Th17 Cells Th22 Cells Th9 Cells

参考图片

Detection of LAP (TGF-beta 1) in Human Platelets by Flow Cytometry. Human platelets were stained with Mouse Anti-Human LAP (TGF-beta 1) Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB2463N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). Histograms were gated on CD41⁺ cells. View our protocol for Staining Membrane-associated Proteins.