| 货号 | IC7437G |
| 别名 | basic leucine zipper transcription factor, ATF-like 3; basic leucine zipper transcriptional factor ATF-like 3; B-ATF-3; FLJ36352; FLJ37535,21 kDa small nuclear factor isolated from T-cells; JDP1; Jun dimerization protein 1; Jun dimerization protein p21SNFT; JUNDM1; SNFT; SNFT21-kD small nuclear factor isolated from T cells | 全称 | Basic Leucine Zipper Transcription Factor, ATF-like 3 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human BATF3 in ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55509 (Human); 381319 (Mouse); 60462 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human BATF3 Met1-Arg127 Accession # Q9NR55 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of BATF3 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB1777A) and either (A) Mouse Anti-Human BATF3 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC7437G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
