| 货号 | IC7476G |
| 别名 | DKFZp762O1912; FLJ14393; MDIG; MDIGDKFZp762O1912; Mina53; MINA53mdig; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC induced nuclear antigen; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; NO52; Nucleolar protein 52 | 全称 | Myc-induced Nuclear Antigen |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human MINA in direct ELISAs.In direct ELISAs, approximately 50%cross-reactivity with recombinant mouse MINA is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84864 (Human); 67014 (Mouse); 266670 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human MINA Met1-Gly192 Accession # Q8IUF8 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | MINA (Myc-induced Nuclear Antigen), also known as Mina53, is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and particularly nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, select epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R, IL-4, and Relm beta, the latter through the influence of TGF-beta. It may exert its regulatory activity through an intrinsic histone demethylase function. Human MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 52-364) that contains a JmjC domain (aa 139-271). There are three potential isoform variants that show either a seven aa substitution for aa 255‑261, an 18 aa substitution for aa 263-465, or a deletion of Glu297. Over aa 2-192, human MINA shares 82% aa sequence identity with mouse MINA. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MINA in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7476G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
