货号 | IC7408G |
别名 | MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; slug homolog, zinc finger protein (chicken); SLUGH; SLUGH1; SLUGzinc finger protein; SNAI2; snail 2; snail homolog 2 (Drosophila); SNAIL2; WS2D; zinc finger protein SNAI2 |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
目标/特异性 | Detects human Slug in direct ELISAs. |
使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 6591 (Human); 20583 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human Slug Met1-His268 Accession # O43623 |
生物活性 | Human |
标记 | Alexa Fluor 488 |
背景 | SLUG, also known as SNAI2, is a zinc-finger transcription factor. SLUG is a transcriptional repressor which may suppress E-cadherin transcription and be involved in an epithelial to mesenchymal transition (EMT). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Slug in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Slug Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7408G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |