Human IL-17 Allophycocyanin MAb (Clone 41809) (100 TESTS)【科瑞奥博】

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Human IL-17 Allophycocyanin MAb (Clone 41809) (100 TESTS)

货号： IC317A 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	IC317A
别名	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
全称	Interleukin 17
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17/IL-17A is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IL-17/IL-17A Ile20-Ala155 Accession # Q16552
生物活性	Human
标记	Allophycocyanin
背景	IL-17/IL-17A is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion Cytokines and Receptors in Tfh Cell Differentiation and Function Development and Characterization of Monoclonal Antibody Clone 41802 to Human IL-17A gamma delta T Cells Helper T (Th) Cells IL-17 Family IL-17 is Produced by T-cells Interactive Pathway: IL-17 Family Signaling Pathways LTi Cells Macrophage Activation Markers Mini-review: Interleukin 17 (IL-17) Multiple Sclerosis and Myelin Related Molecules Natural Killer T (NKT) Cells NCR-ILC3 Neuroinflammation T Cell Cytokine Signaling Technical Note: IL-17 Family Antibodies for Flow Cytometry Th17 Cells Th17 Differentiation: An Evolving Target for Multiple Sclerosis Therapy

参考图片

Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL‑23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human IL‑17/IL‑17A APC‑conjugated Monoclonal Antibody (Catalog # IC317A) and Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.