Human TIM-3 Alexa Fluor 488 MAb (Clone 344823) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TIM-3 Alexa Fluor 488 MAb (Clone 344823) (100 TESTS)

货号： FAB2365G 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	FAB2365G
简介	Clone 344823 was used by HLDA to establish CD designation
别名	CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; Tim-3; TIMD-3; TIMD3KIM-3
全称	T Cell Immunoglobulin Mucin-3
反应种属	Human
应用	Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. Authors: Leeansyah E, Svard J, Dias J, Buggert M, Nystrom J, Quigley M, Moll M, Sonnerborg A, Nowak P, Sandberg J PLoS Pathog, 2015;11(8):e1005072. Species: Human Sample Type: Whole Cells Application: Flow Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection. Authors: Leeansyah E, Ganesh A, Quigley M, Sonnerborg A, Andersson J, Hunt P, Somsouk M, Deeks S, Martin J, Moll M, Shacklett B, Sandberg J Blood, 2013;121(7):1124-35. Species: Human Sample Type: whole cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human TIM‑3 Ser22-Arg200 Accession # Q8TDQ0.2
生物活性	Human
标记	Alexa Fluor 488
背景	TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8⁺ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Sakuishi, K. et al. (2011) Trends Immunol. 32:345. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213. Monney, L. et al. (2002) Nature 415:536. Nakayama, M. et al. (2009) Blood 113:3821. Anderson, A.C. et al. (2007) Science 318:1141. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906. Chiba, S. et al. (2012) Nat. Immunol. 13:832. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093. Ndhlovu, L.C. et al. (2012) Blood 119:3734. Gleason, M.K. et al. (2012) Blood 119:3064. Zhu, C. et al. (2005) Nat. Immunol. 6:1245. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

参考图片

Detection of TIM‑3 in Human PBMCs Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Rat Anti-Human TIM‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2365G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TIM‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Recombinant Human IL‑12 (Catalog # 219-IL) and Goat Anti-Human IL‑4 Polyclonal Antibody (Catalog # AB-204-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Rat Anti-Human TIM‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2365G) or (B) Rat IgG_2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). Dot plots were gated on lymphocytes. View our protocol for Staining Membrane-associated Proteins.