| 货号 | IC3157G |
| 别名 | MGC138165; MGC141991; Protein Wnt-14; wingless-type MMTV integration site family, member 14; wingless-type MMTV integration site family, member 9A; Wnt-14; WNT14protein Wnt-9a | 全称 | Wingless-type MMTV Integration Site Family, Member 9A |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -3a, -6, -7a, -7b, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7483 (Human); 216795 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Wnt‑9a Pro48-Glu92 & Arg231-Phe304 Accession # O14904.2 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Wnt-9a (Wingless integration site family member 9a), formerly Wnt-14, is a 45-47 kDa member of the Wnt family of proteins, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in both embryo and adult. It is found in embryonic mesenchyme adjacent to cartilage, and in lung at the tips of developing bronchioles. In adult, it is found throughout the liver, in hepatocytes, stellate sinusoidal cells, biliary epithelium and endothelium. It also appears in colonic epithelium and in many tumor cell types. It would appear that Wnt-9a binds to Fzd-7 and -9. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Wnt‑9a in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Wnt‑9a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3157G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
