| 货号 | IC5980G |
| 别名 | complement factor H-related protein 4; CFHL4; CFHR4; complement factor H-related 4; FHR4; FHR-4 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CFHR1, rhCFHR2, or rhCFHR5 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10877 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4 Glu20-Glu331 Accession # Q92496 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | FHR4 (Complement factor H-related protein 4), also known as CFHR4, is an 84-86 kDa glycoprotein member of group 2 in the factor H-related protein family of molecules. It is synthesized by hepatocytes and circulates in plasma as a monomer. FHR4 binds to lipoproteins (LDL and VLDL), C3b and C3d, and to native (pentameric) C-reactive protein (pCRP). In addition to full-length 86 kDa CFHR4 (called FHR4A), there are two alternative splice forms. One simply shows a deletion of Glu20 relative to the full-length form. A second called FHR4B is 42-45 kDa in size, and shows a deletion of amino acids (aa) 60-306 within the mature CFHR4 molecule. This short isoform is the molecule used as the immunogen for clone 640212. This 312 aa short form contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. Both major isoforms appear to show the same activity. That is, they serve as a platform for C3 convertase assembly, and bind pCRP which leads to classical complement activation. Over aa 20-331, human CFHRB shares 47% aa identity with a related molecule found in rat. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Complement Factor H‑related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Complement Factor H‑related 4/CFHR4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5980G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Intracellular Molecules. |
