| 货号 | IC6456G |
| 别名 | cytokine receptor; EBI3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL27B; IL-27B; interleukin-27 subunit beta | 全称 | Epstein-Barr virus induced gene 3 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10148 (Human); 50498 (Mouse); 680609 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit Arg21-Lys229 (predicted) Accession # Q14213 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | IL-27 is a heterodimeric cytokine comprised of the IL-12 family that is composed of two chains, EBV-induced gene 3( EBI3), a 33 kDa glycosylated protein homologous to the p40 subunit of IL-12, and p28, a 28 kDa peptide analogous to the p35 subunit of IL-12. IL-27 has been found to be expressed by activated monocytes and monocyte-derived dendritic cells, astrocytes, adipocytes, CD4+ T cells, and macrophages. In human tissues, EBI3 and p28 coexpression has been observed in macrophages or macrophage-derived cells, and in endothelial cells. The IL-27 receptor complex comprises two chains, IL-27R (also called WSX-1 or TCCR) and gp130. IL-27 is the only known ligand for IL-27R, whereas gp130 is a receptor component of several cytokines of the IL-6 family. Functionally, IL-27 appears to be immunosuppressive. On T cells in mice, it induces IL-10 secretion while suppressing IL-17A and GM-CSF production. But the relationship between IL-27 and IL-17A may be complex. After IL-17A induction, IL-27 secretion occurs in unstimulated cells, suggesting IL-27 downregulates bystander activation in inflammatory conditions. EBI3 also complexes with p35 (IL-12) to form IL-35. The mature EBI3 sequences for human and mouse share 62% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of IL‑27/IL‑35 EBI3 Subunit in IL-18-treated KG‑1 Human Cell Line by Flow Cytometry. KG‑1 human acute myelogenous leukemia cell line, resting (open histogram) or treated with rhIL-18 (filled histogram) was stained with Mouse Anti-Human IL‑27/IL‑35 EBI3 Subunit Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6456G) or isotype control antibody (Catalog # IC002G). View our protocol for Staining Intracellular Molecules. |
