| 货号 | FAB81751G |
| 别名 | antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5; CD228 antigen; CD228; FLJ38863; MAP97; MAP97Melanoma-associated antigen p97; melanotransferrin; membrane-bound transferrin-like protein; MFI2; MGC4856; MTF; MTF1; p97 |
| 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Melanotransferrin/CD228 in direct ELISAs. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4241 (Human); 30060 (Mouse); 288038 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human Melanotransferrin/CD228 Met1-Gly711 Accession # P08582 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Melanotransferrin, also known as MTF, CD228, Melanoma-associated Antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is synthesized as a 738 amino acid (aa) protein. Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Melanotransferrin/CD228 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Melanotransferrin/CD228 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB81751G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
