| 货号 | FAB633G |
| 别名 | CD264 antigen; CD264; DcR2; DCR2decoy with truncated death domain; Decoy receptor 2; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TRAIL-R4; TRAILR4TRAIL receptor 4; TRUNDDTRAIL receptor with a truncated death domain; tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain | 全称 | TRAIL Receptor 4 |
| 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human TRAIL R4/TNFRSF10D in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL, rhTNF‑ alpha, and rhTNF-beta is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8793 (Human) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D Ala56-His211 Accession # Q9UBN6 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | TRAIL-R4, also known as TRAIL-R4 alpha, Decoy Receptor 2 (DcR2) and TNFRSF10D,is a 39-48 kDa member of the TNFR Superfamily of proteins. Mature TRAIL-R4/DcR2 is a type Itransmembrane protein 331 amino acids (aa) in length. It contains a 156 aa extracellular domain(ECD) (aa 56-211), and a 154 aa cytoplasmic region that contains a truncateddeath domain. The presence of thistruncation is not believed to allow for a positive signal for the initiation ofapoptosis. This has led to its descriptionas a "decoy receptor", suggesting that its function is to simply sequester and decreasethe amount of TRAIL available for binding to the two apoptosis-inducing TRAILreceptors, DR4 and DR5. At this time,this view would appear to be unlikely, as it now seems that DcR2 actuallycomplexes with DR5/TRAIL-R2, blocking the activation of Caspase-8, a first stepin the initiation of apoptosis. Inaddition, in a ligand-dependent manner, the presence of DcR2 has also beenassociated with the increased survival of select leukocytes. Notably, an isoform variant for TRAIL-R4(called TRAIL-R4 beta ) has been reported that shows an absence of aa 87-124, adeletion that would render it incapable of binding to TRAIL. Cells reported to express TRAIL-R4 arevaried, and include eosinophils, NKT cells, CD8+ (but not CD4+) T cells, coloniccolumnar epithelium, neutrophils, endometrial columnar epithelium, testicularspermatids and testosterone-secreting Leydig cells, and multiple tumor celltypes. A rodent counterpart to TRAIL-R4has not been reported. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TRAIL R4/TNFRSF10D in Human Whole Blood Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Mouse Anti-Human TRAIL R4/TNFRSF10D Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB633G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
