| 货号 | IC8999R-100 |
| 别名 | CD288 antigen; CD288; MGC119599; MGC119600; TLR8; toll-like receptor 8 | 全称 | Toll-like Receptor 8 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human TLR8 in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51311 (Human); 170744 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human TLR8 Glu27-Thr827 Accession # Q9NR97 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 647 |
| 背景 | TLR8, also designated as CD288 (cluster of differentiation 288),is a 135-140 kDa member of the Toll-like Receptor (TLR) family, TLR7/8/9 subfamily. TLRs make up a family of pattern recognition receptors that playimportant roles in the innate immune response. Broad classes of pathogens (e.g.viruses, bacteria, and fungi) constitutively express a set ofmutation-resistant molecules called pathogen-associated molecular patterns(PAMPs). These microbial molecular markers may be composed of proteins,carbohydrates, lipids, nucleic acids and/or combinations thereof. IndividualTLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascadesthat promote the immune response. Cells known to express TLR8 include dermal and myeloid dendritic cells, monocytes, CD8+ T cells and colonic columnar epithelium. Structurally, TLRs are type I transmembranereceptors that possess varying numbers of extracellular N-terminal leucine-richrepeat (LRR) domains, followed by a cysteine-rich region, a TM segment, and anintracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the largerIL-1 R/TLR superfamily. Human TLR8 is a homodimeric endosomal receptor that recognizes singlestranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza,Sendai, and Coxsackie B viruses. TLR8 binding to the viral RNA recruits MyD88and leads to activation of the transcription factor NF-kB and an antiviralresponse. Over amino acids (aa) 27-827, human and mouse TLR8 share 67% amino acid sequence identity. Notably, rodent TLR8 does not posess an RQSYA sequence between aa 438-442, rendering it unresponsive to nucleic acid sequences that lack an accompanying poly-thymidine segment. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TLR8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TLR8 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8999R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
