Human Factor III/Tissue Factor Alexa Fluor 488 (Cl 323519) (100 TESTS)【科瑞奥博】

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Human Factor III/Tissue Factor Alexa Fluor 488 (Cl 323519) (100 TESTS)

货号： FAB23391G 基本售价： 4521.0 元规格： 100 Tests

产品信息

概述
货号	FAB23391G
别名	CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
反应种属	Human
应用	Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2152 (Human); 14066 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III Gly34-Glu251 Accession # P13726
生物活性	Human
标记	Alexa Fluor 488
背景	Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa integral membrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and as a soluble form, plays an important coagulation-independent role in processes such as angiognesis (2-4). Synthesized as a 295 amino acid (aa) precursor, TF consists of a signal peptide (aa 1-32) and the mature chain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (5-8). TF is found on endothelial cells, monocytes and vascular smooth muscle cells. Over aa 33-251, human and mouse TF share 58% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. Gedding, J.E. and N. Mackman (2014) Thromb. Haemost. 111:570. Hobbs, J.E. et al. (2007) Thromb. Res. 120Suppl 2:S13. Scarpati, E.M. et al. (1987) Biochemistry 26:5234. Fisher, K.L. et al. (1987) Thromb. Res. 48:89. Morrissey, J.H. et al. (1987) Cell 50:129. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

参考图片

Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB23391G) or (B) Mouse IgG₁ Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.