Human TLR9 Alexa Fluor 488 Affinity Purified Polyclonal Ab (100 TESTS)【科瑞奥博】

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Human TLR9 Alexa Fluor 488 Affinity Purified Polyclonal Ab (100 TESTS)

货号： IC7108G 基本售价： 4521.0 元规格： 100 Tests

产品信息

概述
货号	IC7108G
别名	CD289 antigen; CD289; toll-like receptor 9
全称	Toll-like Receptor 9
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human TLR9 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TLR9 is observed, and less than 1% cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR3, rhTLR4, rhTLR5, rhTLR7, rhTLR8, and rhTLR10 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	54106 (Human); 81897 (Mouse); 338457 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TLR9 Asn64-Glu189 Accession # Q9NR96
生物活性	Human
标记	Alexa Fluor 488
背景	TLR9 (Toll receptor 9; also CD289) is a 145-150 kDa member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium, CD123⁺ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that exhibit either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse). TLR9 is found in the ER, and translocates to either the cell membrane, or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032 amino acids (aa) in length. It possesses a 793 aa extracellular region that contains 26 LRRs (aa 26-818), plus a 193 aa cytoplasmic domain. The full-length 150 kDa form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80 kDa cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa 2-16, a second possesses an alternate start site at Met58, while a third and fourth show alternative start sites aa 23 and 24 upstream of the standard site. Over aa 64-189, human TLR9 shares 76% aa identity with mouse TLR9.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Activation BIObrief: Pattern Recognition Receptors & the Innate Immune Response Human CD Marker Products IL-1 Family Innate Immunity and the Toll-like Receptor Family Interactive Pathway: Inflammasome Activation Pathways Interactive Pathway: Toll-Like receptor Signaling Pathways Mini-review: The Toll-like Receptor Family Neuroinflammation NFkB Pathway NOD-like Receptors and the Inflammasome Parturition & Surfactant Protein A Pattern Recognition Receptors Plasmacytoid Dendritic Cells TLR4: Contributing to Metabolic Syndrome in Multiple Tissues Toll-Like Receptors

参考图片

Detection of TLR9 in Human CD123⁺ PBMCs by Flow Cytometry. Human CD123⁺ peripheral blood mononuclear cells (PBMCs) were stained with Sheep Anti-Human TLR9 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC7108G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.