| 货号 | FAB8140G |
| 别名 | D6Bwg0781e; Img; LIG1; LIG-1 | 全称 | Leucine-rich Repeats and Immunoglobulin-like Domains 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human LRIG1 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse LRIG1 and and less 1% cross-reactivity with recombinant human LRIG3 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26018 (Human); 16206 (Mouse); 312574 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human LRIG1 Ala35-Ser779 Accession # Q96JA1 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | LRIG1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 134-145 kDa glycoprotein that belongs to the LRIG gene family. It is widely expressed, and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons. LRIG1 is believed to negatively regulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number. Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction. Mature human LRIG1 is a 1059 amino acid (aa) type I transmembrane protein. It contains a large 760 amino acid (aa) extracellular domain (ECD) (aa 35-794) plus a 278 aa cytoplasmic region. The ECD contains 17 LRRs (aa 35‑491) and three C2-type Ig-like domains (aa 495-780). These two domain types are each sufficient for EGFR binding. There are two potential alternative splice forms. One contains a 27 aa insertion after Gly874, while another shows a 24 aa insertion after Lys387 coupled to a Gln substitution for aa 644-691. The LRIG1 ECD undergoes proteolysis, generating 100-110 and 55-60 kDa soluble fragments. Over aa 35‑779, human LRIG1 shares 90% aa sequence identity with mouse LRIG1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LRIG1 in LNCaP Hman Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Sheep Anti-Human LRIG1 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8140G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
