| 货号 | FAB6484G |
| 别名 | Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA | 全称 | Paired-Ig-likeType 2 Receptor alpha |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human PILR-alpha in direct ELISAs. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29992 (Human); 231805 (Mouse); 288568 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human PILR-alpha Gln20-Thr196 Accession # Q9UKJ1 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | PILR-alpha (Paired Immunoglobulin-like, type 2 Receptor alpha) is a monomeric 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by neutrophils, monocytes, macrophages, CD14+CD1a- DC, corneal epithelium and retinal pigment cells. It is known to bind to CD99, PANP, Collectin-12, and NPDC-1. The target for PILR-alpha appears to be sialylated O-glycans. PILR-alpha also acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and may either show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of PILR‑ alpha in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR‑ alpha Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6484G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
