| 货号 | FAB100T-100 |
| 别名 | CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York). |
| 使用方法 | Flow Cytometry: 5 µL/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 916 (Human); 12503 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human thymocytes (1) |
| 生物活性 | Human |
| 标记 | Alexa Fluor 594 |
| 背景 | CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD3 epsilon Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB100T) or (B) Mouse IgG1 Alexa Fluor 594 Isotype Control (Catalog # IC002T). View our protocol for Staining Membrane-associated Proteins. | |
CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Alexa Fluor® 594‑conjugated Monoclonal Antibody (red; Catalog # FAB100T) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
