| 货号 | FAB51051G |
| 别名 | Nerve injury-induced protein 1; nerve injury-induced protein-1; NIN1; NINJ1; ninjurin 1; NINJURIN; ninjurin-1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human Ninjurin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinanthuman Ninjurin-2 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4814 (Human); 18081 (Mouse); 25338 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Ninjurin-1 Asp2-Val81 Accession # Q92982 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Ninjurin-1 (Nerve Injury-induced Protein 1) is a 20-22 kDa phosphoprotein member of the Ninjurin family of transmembrane (TM) proteins. It is either constitutively or inducibly expressed by Schwann cells, PNS neuron satellite cells, neutrophils, endothelial cells, macrophages, podocytes and hepatocytes, and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. With respect to human Ninjurin-2, which has only 65 aa in it N-terminal ECD, human Ninjurin-1 shares 51% aa sequence identity with human Ninjurin-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Ninjurin‑1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Ninjurin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB51051G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
