| 货号 | FAB6700A |
| 别名 | BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human CD160 in direct ELISAs. In direct ELISAs and Western blots, nocross-reactivity with recombinant mouse CD160 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11126 (Human); 54215 (Mouse); 502585 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human CD160 Ile27-Ser159 Accession # O95971 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules. It is expressed on select hematopoietic cell types, including CD56dimCD16+ cytotoxic NK cells, CD8+CD28- effector T cells, δ/ gamma T cells, and restricted CD4+ T cells. It is a receptor for HLA-C molecules, and its engagement induces CD160+ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program. Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181). It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V‑type Ig-like domain (aa 27-122). CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182. The TM form appears to have a splice variant that lacks aa 25-133. Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of CD160 in Human PBMCs byFlow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-Human CD160APC‑conjugated Monoclonal Antibody (Catalog # FAB6700A) and MouseAnti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody(Catalog # FAB2408P).View our protocol for StainingMembrane-associated Proteins. |
