| 货号 | IC4937G |
| 别名 | ICRF; PSP; PTP | 全称 | Regenerating Islet-derived 1A |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human Reg1A in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Reg1B, rhReg4, recombinant rat (rr) Reg2, or rrReg3. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5967 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Reg1A Gln23-Asn166 Accession # P05451 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Human Reg1A, also known as Reg1 alpha, PTP, PSP and Lithostathine, is a 16-22 kDa, variably glycosylated member of the Reg (Regenerating) family, C-type lectin superfamily of molecules. It is further classified as a type I subfamily member based on gene organization and expression pattern. These are four Reg subfamilies spread across multiple species, with three (or four) additional human Reg family genes assigned to three of the subfamilies. Reg1A is secreted, and circulates as either a monomer, dimer, or tetramer. Once secreted, Reg1A may undergo proteolysis with removal of the N-terminal 11 amino acids (aa). This renders it insoluble with subsequent fibril formation. Reg1A is typically inducible, and human cells reported to express Reg1A include pancreatic islet beta -cells, neurons, intestinal Paneth cells, stomach Chief cells, cardiomyocytes, salivary duct epithelium and antral endocrine cells of the stomach. Reg1A has proliferative effects on multiple cell types, and likely serves as an anti-inflammatory agent. This is presumably mediated by binding to its receptor, EXTL3. Mature human Reg1A (aa 23-166) shares 75%, 69% and 88% aa sequence identity with mouse RegI,rat Reg, and human Reg1B, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Reg1A in AGS Human Cell Line by Flow Cytometry. AGS human gastric adenocarcinoma cell line was stained with Rat Anti-Human Reg1A Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4937G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
