| 货号 | FAB3035N |
| 别名 | ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse EphA4, A5, A6, A7, A8, or recombinant rat EphB1 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1969 (Human); 13836 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human EphA2 Gln25-Asn534 Accession # P29317 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | EphA2 (Erythropoietin-producing Hepatocellular Carcinoma-A2), also known as Eck, Myk2, and Sek2, is a 120-130 kDa member of the Ephrin receptor subfamily, tyrosine protein kinase family of molecules. It is one of at least 13 mammalian subfamily members that are divided into two groups (A and B) that are named for the corresponding class of ligand (Ephrins [Eph-receptor Interacting Protein]) to which they bind (1, 2). Mature EphA2 is a 953 amino acid (aa) type I transmembrane glycoprotein that contains a 514 aa extracellular domain (ECD). The ECD is characterized by the presence of one Eph ligand-binding domain and two fibronectin type III repeats. EphA2 binds Ephrins A1-A5, with subsequent receptor oligomerization and signal transduction (1, 2). It also exists, however, in a ligand-independent, auto-activated monomeric and inactive, homodimeric state, leading to complexities in its actions (3). Further, it is also known to form heterodimers with EphB6, a kinase-negative receptor that dampens EphA2 activity (4). EphA2 is expressed by a wide variety of cell types, including osteoblasts, neurons, capillary endothelium, Schwann cells, keratinocytes, Langerhans cells (LC) and IDC (mature LCs), cortical lens fiber cells and placenta-derived mesenchymal stem cells where EphA2 may serve as a biomarker (2, 5-11). Over aa 25-534, human and mouse EphA2 share 91% sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human EphA2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3035N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins. |
