Human NKG2D Allophycocyanin MAb (Clone 149810) (100 TESTS)【科瑞奥博】

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Human NKG2D Allophycocyanin MAb (Clone 149810) (100 TESTS)

货号： FAB139A-100 基本售价： 4521.0 元规格： 100 Tests

产品信息

概述
货号	FAB139A-100
简介	Clone 149810 was used by HLDA to establish CD designation
别名	CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
全称	Natural Killer G2D
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human NKG2D/CD314 in Western blots.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	22914 (Human); 27007 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S J Immunol, 2014;193(4):1654-65. Species: Human Sample Type: Whole Cells Application: Flow TL1A induces TCR independent IL-6 and TNF-alpha production and growth of PLZF(+) leukocytes. Authors: Reichwald K, Jorgensen T, Tougaard P, Skov S PLoS ONE, 2014;9(1):e85793. Species: Human Sample Type: Whole Cells Application: Flow Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells. Authors: Markel G, Seidman R, Besser MJ PLoS ONE, 2009;4(5):e5597. Species: Human Sample Type: Whole Cells Application: Flow CD27 defines phenotypically and functionally different human NK cell subsets. Authors: Vossen MT, Matmati M, Hertoghs KM, Baars PA, Gent MR, Leclercq G, Hamann J, Kuijpers TW, van Lier RA J. Immunol., 2008;180(6):3739-45. Species: Human Sample Type: Whole Cells Application: Flow 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells. Authors: Kim YJ, Han MK, Broxmeyer HE Blood, 2007;111(3):1378-86. Species: Human Sample Type: Whole Cells Application: Flow Phosphostim-activated gamma delta T cells kill autologous metastatic renal cell carcinoma. Authors: Viey E, Fromont G, Escudier B, Morel Y, Da Rocha S, Chouaib S, Caignard A J. Immunol., 2005;174(3):1338-47. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
生物活性	Human
标记	Allophycocyanin
背景	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.
运输条件	Blue Ice
存放说明	4℃
参考文献	Li, P. et al. (2001) Nature Immunol. 2:443. Steinle, A. et al. (2001) Immunogenetics 53:279. Cosman, D. et al. (2001) Immunity 14:123. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158. Wu, J. et al. (1999) Science 285:730. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150. Groh, V. et al. (2001) Nature Immunol. 2:255. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521. Diefenbach, A. et al. (2001) Nature 413:165. NKG2D and its Ligands (2002) www.RnDSystems.com.

参考图片

Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3^- cells were stained with Mouse Anti-Human
NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 APC‑conjugated Monoclonal Antibody (Catalog # FAB139A) or (B) Mouse IgG₁ APC Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.