| 货号 | FAB4900G |
| 别名 | FLJ23907; MGC45780; scavenger receptor class A, member 5 (putative); Tesr; testis expressed scavenger receptor; UNQ2938/PRO28700 | 全称 | Scavenger Receptor Class A Member 5 |
| 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human SCARA5 in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant mouse SCARA5 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 286133 (Human); 71145 (Mouse); 305974 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human SCARA5 Arg83-His495 Accession # Q6ZMJ2 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Scavenger Receptor A5 (SCARA5), also known as Tesr, is a 70-75 kDa kDa member of the scavenger receptor "supergroup" of molecules. It is one of at least six class A members that possess a collagen-like domain and form intramembrane homotrimers (1,3). Class A scavenger receptors are type II transmembrane proteins. Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a Scavenger Receptor Cysteine-Rich (SRCR) domain (3-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. Among other class A members, it shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1 in the ECD. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of 200-210 kDa (5,7). It is highly expressed by a number of cell types, including testicular Sertoli cells, transitional epithelium, respiratory pseudostratified columnar epithelium, intestinal M cells, astrocytes, retinal ganglion cells, endothelial cells and likely intestinal Paneth cells (5, 8). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5). It is also recognized to bind and transport L (light)-ferritin across anatomical boundaries (8). Finally, SCARA5 has also been found in both nucleus and cytoplasm (8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB4900G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
