Human MMP-14/MT1-MMP Alexa Fluor 488 MAb (Clone 128527) (100 TESTS)【科瑞奥博】

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Human MMP-14/MT1-MMP Alexa Fluor 488 MAb (Clone 128527) (100 TESTS)

货号： FAB9181G 基本售价： 4521.0 元规格： 100 Tests

产品信息

概述
货号	FAB9181G
别名	EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1
全称	Matrix Metalloproteinase 14/Membrane Type 1 MMP
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4323 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. NEDD9/Arf6-dependent endocytic trafficking of matrix metalloproteinase 14: a novel mechanism for blocking mesenchymal cell invasion and metastasis of breast cancer. Authors: Loskutov Y, Kozyulina P, Kozyreva V, Ice R, Jones B, Roston T, Smolkin M, Ivanov A, Wysolmerski R, Pugacheva E Oncogene, 2015;34(28):3662-75. Species: Human Sample Type: Whole Cells Application: IHC Not Specified
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP Tyr112-Ala541 (predicted) Accession # P50281
生物活性	Human
标记	Alexa Fluor 488
背景	MMP-14/MT1-MMP (Matrix Metalloproteinase 14) is a member of the peptidase M10A family of enzymes. As such, it is one of some 25 zinc-dependent endopeptidases that are either soluble or membrane-bound. MMP-14 is expressed as a 63-64 kDa precursor that undergoes processing to generate either a 60 kDa catalytically-active transmembrane form, or a 55 kDa active soluble form. A number of substrates are reported for MMP-14, including Collagen I, II, and III, Laminin, Endoglin, Muc-1, Pro-MMP-13 and -2, and CD44. Molecules suggested to inhibit MMP-14 include TIMP-2, -3, and -4. Cells known to express MMP-14 include monocytes, platelets, osteoclasts, endothelial cells, fibroblasts, and muscle satellite cells. Over amino acids (aa) 112-284, human MMP-14 shares 98% aa sequence identity with mouse MMP-14.
运输条件	Blue Ice
存放说明	4℃
参考文献	Collagen Modifying Enzymes Extracellular Matrix Remodeling and Cell Migration During EMT ICC / IHC Images: MMP-14/MT1-MMP Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression

参考图片

Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9181G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.