Human SOX17 Allophycocyanin Affinity Purified Polyclonal Ab (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human SOX17 Allophycocyanin Affinity Purified Polyclonal Ab (100 TESTS)

货号： IC1924A 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	IC1924A
别名	FLJ22252; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
全称	Transcription Factor SOX17
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	64321 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet Nat Protoc, 2015;10(3):363-81. Species: Human Sample Type: Whole Cells Application: Flow BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages. Authors: Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA Cell Stem Cell, 2010;9(2):144-55. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SOX17 Asp177-Val414 Accession # Q9H6I2
生物活性	Human
标记	Allophycocyanin
背景	SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kanai-Azuma, M. et al. (2002) Development 129:2367. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153. Sohn, J. et al. (2006) J. Neuroscience 26:9722.

参考图片

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.