| 货号 | FAB6647P |
| 别名 | CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP receptor; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4 | 全称 | Macrophage Stimulating Protein Receptor |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross‑reactivity with recombinant mouse MSP R is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4486 (Human); 19882 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human MSP R/Ron Glu25-Ser956 Accession # Q04912 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | RON (Recepteur dOrigine Nantais [Receptor originating (ordiscovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a180-190 kDa member of the tyrosine kinase family, protein kinase superfamily ofmolecules. Human mature RON is a type Itransmembrane heterodimeric glycoprotein that arises from the proteolytic cleavageof a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a35-40 kDa a-chain(aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and thetransmembrane segment appears over aa 958-978. Multiple cell types are known to express RON, and these includeosteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized andnonkeratinized stratified squamous epithelium, intestinal Paneth cells, renalproximal tubule and mesangial cells, monocytes, neutrophils and mammaryepithelium. On the cell surface, RONserves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization andreceptor signaling. In addition, RON isalso known to heterodimerize with multiple integral membrane proteins, eitherindividually or as part of a complex. Proteins identified to date include b1 integrins, c-MET/HGFR,E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, andCD44v6. RON may also be foundintracellularly, where it interacts with either the androgen receptor or EGFRin the cytoplasm, or in the nucleus, where it acts as a transcription factorcoupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There areabout a dozen alternative splice forms for RON, some soluble, while others aretransmembrane, and either constitutively active or biochemically inert. Theyhold the potential to interact with full-length RON and impact its signalingcapabilities. Reflecting the diversenature of RON-expressing cells, RON has been found to generate multipleoutcomes, including the promotion of epithelial-to-mesenchymal transitions, themaintenance of M2 macrophage phenotypes, and the initiation of keratinocyteproliferation and migration. Over aa25-956, human and mouse RON share 76% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MSP R/Ron in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human MSP R/Ron PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6647P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
