Human Phospho-p53 (S15) Phycoerythrin MAb (Clone 261352) (100 TESTS)
货号：
IC1839P 基本售价：
4382.2 元 规格：
100 Tests
产品信息
概述| 货号 | IC1839P |
| 别名 | Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects human p53 that is phosphorylated at S15 in Western blots. In Western blots, no cross-reactivity with human p53 that is unphosphorylated at S15 is detected. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 7157 (Human); 22059 (Mouse); 24842 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Phosphopeptide containing the human p53 S15 site
Accession # P04637 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | p53 is a 52-55 kDa member of the p53 family ofproteins. It is ubiquitously expressed,being found in both cytoplasm (as a monomer) and nucleus (as anoligomer/tetramer). Human p53 is 393amino acids in length and contains multiple domains that interact with multiplebinding partners. There are numerousposttranslational modification sites, including multiple phosphorylation,acetylation, NEDDylation and ubiquitination sites, plus one SUMOylation and twomethylation sites. N-terminalmodifications tend to stabilize p53, while C-terminal modifications impactmolecule oligomerization, DNA-binding and ubiquitination. Phosphorylation on Ser15 inhibits MAM2modification of p53, thereby extending its half-life. Notably, there are nine possible p53 isoforms,six of which do not contain Ser15 of the standard (or full-length) form. Thus, they are unlikely to show strong positivestaining that is associated with the phosphorylation of Ser15. p53 is best known as a preserver of cellgenome integrity. In the nucleus, it stimulates needed repairs to DNA and, ifnecessary, cease cell division and/or initiate apoptosis. In addition, it has at least one role in thecytoplasm where it is known to regulate glucose consumption. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Apoptosis Transcription Factors and Regulators
- Cancer Biomarkers
- Cancer Stem Cell Transcription Factors
- Cell Cycle
- Checkpoint
- DNA Damage and Repair
- Epithelial Stem Cell Transcription Factors
- Hematopoietic Stem Cell Transcription Factors and Regulators
- ICC / IHC Images: p53
- Immunoglobulin-Like Domain Transcription Factors
- Mesenchymal Stem Cell Transcription Factors
- Mini-review: DNA Damage Response
- Mini-review: Genomic Instability Syndromes
- Neural Stem Cell Transcription Factors
- p53 Pathway
- p53: More than a Transcription Factor?
- Parkinsons Disease Related Molecules
- Pluripotent Stem Cell Transcription Factors
- TNF Superfamily Regulators
- Transcription Factors and Regulators
- Transcription Factors in the Akt Pathway
|
参考图片Detection of p53 in Camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was treated with Camptothecin overnight (filled histogram) or untreated (open histogram), then stained with Mouse Anti-Human Phospho-p53 (S15) PE‑conjugated Monoclonal Antibody (Catalog # IC1839P) or isotype control antibody (Catalog # IC002P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
