Human SOX17 Alexa Fluor 488 MAb (Clone 614013) (100 TESTS)【科瑞奥博】

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Human SOX17 Alexa Fluor 488 MAb (Clone 614013) (100 TESTS)

货号： IC19241G 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	IC19241G
别名	FLJ22252; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
全称	Transcription Factor SOX17
反应种属	Human
应用	Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human SOX17 in direct ELISAs.
使用方法	Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	64321 (Human)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human SOX17 Asp177-Val414 Accession # Q9H6I2
生物活性	Human
标记	Alexa Fluor 488
背景	SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kanai-Azuma, M. et al. (2002) Development 129:2367. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

参考图片

SOX17 in BG01V Human Embryonic Stem Cells. SOX17 was detected in immersion fixed definitive endoderm-differentiated BG01V human embryonic stem cells using Mouse Anti-Human SOX17 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green, left panel; Catalog # IC19241G) at 10 µg/mL for 3 hours at room temperature and counterstained with DAPI (blue, right panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human SOX17 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC19241G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.