| 货号 | FAB332A-100 |
| 别名 | CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein | 全称 | Stem Cell Factor Receptor |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human CD117/c-kit in ELISAs and Western blots. In sandwich immunoassays and Western blots, no cross-reactivity with recombinant mouse CD117 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3815 (Human); 16590 (Mouse); 64030 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kit Gln26-Thr520 Accession # P10721 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | Stem Cell Factor Receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, Stem Cell Factor (SCF), also known as c-kit Ligand, Mast Cell Growth Factor, play essential roles in gametogenesis, melanogenesis, and hematopoiesis. The human SCF Receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment, and a 429 aa residue cytoplasmic domain. SCF Receptor is a member of the type III subfamily of Receptor Tyrosine Kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF Receptor is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF Receptor expression has also been reported in a number of human tumor cell lines. SCF Receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF Receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF Receptor binds SCF with high affinity and is a potent SCF antagonist. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD117/c-kit in TF‑1 Human Cell Line by Flow Cytometry. TF‑1 human erythroleukemic cell line was stained with Mouse Anti-Human CD117/c‑kit APC‑conjugated Monoclonal Antibody (Catalog # FAB332A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
