| 货号 | IC3201P |
| 别名 | C-C motif chemokine 11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human CCL11/Eotaxin in Western blots. Does not cross-react with recombinant cotton rat CCL3, 4,5, recombinant human CCL1, 3, 4, 5, 8, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17,19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6356 (Human); 20292 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Multiple beta 1 integrins mediate enhancement of human airway smooth muscle cytokine secretion by fibronectin and type I collagen. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human CCL11/Eotaxin Accession # P51671 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | CCL11, also known as Eotaxin, is a secreted potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 is an O-glycosylated, 13 kDa peptide whose cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate a 74 amino acid residue mature human CCL11. At the amino acid sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. CCL11 circulates as a monomer, homodimer and heterodimer in a complex with either CCL2 or CCL8. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptors 3 and 5, plus CXCR3 have now been identified to be specific receptors for human CCL11. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibits infection by the HIV isolates. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CCL11/Eotaxin in WS‑1 Human Cell Line by Flow Cytometry. WS‑1 human fetal skin fibroblast cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA) and Recombinant Human IL‑4 (Catalog # 204-IL)was stained with Mouse Anti-Human CCL11/Eotaxin PE‑conjugated Monoclonal Antibody (Catalog # IC3201P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
