Human B7-2/CD86 Alexa Fluor 594 MAb (Clone 37301) (100 TESTS)
货号：
FAB141T-100 基本售价：
4382.2 元 规格：
100 Tests
产品信息
概述| 货号 | FAB141T-100 |
| 别名 | Activation B7-2 antigen; B70; B7-2 antigen; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells)
|
| 目标/特异性 | Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 942 (Human); 12524 (Mouse); 56822 (Rat) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 594 |
| 背景 | B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon-gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Acute Lymphoblastic Leukemia
- B Cell Activation
- B Cell Markers
- Common Macrophage Markers
- Control of Immune Responses via CD28 and CTLA-4
- Co-stimulatory and Co-inhibitory Molecules
- Co-stimulatory Molecules Associated with Tfh Cells
- CTLA-4: Hiding in Plain Sight
- Dendritic Cell Co-stimulatory Molecules
- Dendritic Cell Development
- Flow Cytometry & Cell Selection/Detection
- Human CD Marker Products
- ICC / IHC Images: B7-2/CD86
- Macrophage Activation Markers
- Mini-review: B7 & CD28 Superfamilies
- Mini-review: B7 & CD28/CTLA-4 Receptor Families
- Mini-review: Dendritic Cell Maturation
- Mini-review: Immune Tolerance
- Mini-review: Th1/Th2
- Multiple Myeloma
- Myeloid-derived Suppressor Cells (MDSC)
- Regulatory T Cells (Tregs)
- T Cell Antigen Recognition
- Technical Notes: B7 Family Research Tools
|
参考图片Detection of B7‑2/CD86 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human B7‑2/CD86 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB141T) or (B) Mouse IgG 1 Alexa Fluor 594 Isotype Control (Catalog # IC002T). View our protocol for Staining Membrane-associated Proteins. |
