Human Granzyme B Phycoerythrin MAb (Clone 351927) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Granzyme B Phycoerythrin MAb (Clone 351927) (100 TESTS)

货号： IC2906P 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	IC2906P
别名	CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3002 (Human); 14939 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51. Authors: Dorneles E, Lima G, Teixeira-Carvalho A, Araujo M, Martins-Filho O, Sriranganathan N, Al Qublan H, Heinemann M, Lage A PLoS ONE, 2015;10(9):e0136696. Species: Bovine Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Granzyme B Gly19-Tyr247 Accession # P10144
生物活性	Human
标记	Phycoerythrin
背景	Granzyme B is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of Cytotoxic T Lymphocytes (CTL) and Natural Killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various Caspases and cleaves proteins such as Aggrecan (3). Human Granzyme B is synthesized as a 247 amino acid (aa) precursor with a signal peptide (aa 1-18), a pro peptide (aa 19-20), and a mature chain (aa 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of aa 19-247 was expressed and purified. After being activated by active Cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.

参考图片

Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry. NK‑92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PE‑conjugated Monoclonal Antibody (Catalog # IC2906P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.