| 货号 | IC935P |
| 别名 | ADAM 15; ADAM metallopeptidase domain 15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin | 全称 | A Disintegrin and Metalloprotease-like Domain 15 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8751 (Human); 11490 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15 Asp207-Thr696 Accession # Q13444 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | ADAM15, also known as MDC15, Disintegrin and Metallo- and Proteinase Domain-containing Protein 15, and Metargidin, is a member of a family of transmembrane proteins that containdisintegrin and metalloprotease domains. It is synthesized as a 110 kDa, type I transmembrane proform that may be processed into a 75 kDa form that contains the metalloprotease plus disintegrin domain. Further processing may release the 26 kDa metalloproteinase domain, extending proteinase activity beyond the cell membrane. Members of this family havebeen implicated in cell adhesion via integrin binding and shedding ofcell surface molecules. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ADAM15 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ADAM15 Ectodomain PE‑conjugated Monoclonal Antibody (Catalog # IC935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
