| 货号 | IC13292P |
| 别名 | beta 1 (88kD); beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human, mouse, and rat beta -Catenin in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1499 (Human); 12387 (Mouse); 84353 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human beta -Catenin Ala2-Leu781 Accession # P35222 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | beta -Catenin is a 94-96 kDa member of the beta -Catenin family, armadillo-repeat superfamily of intracellular molecules. Human beta -Catenin is 781 amino acids (aa) in length and contains three basic regions; there is a "flexible" N-terminus (aa 1-110) that undergoes phosphorylation, O-linked glycosylation, acetylation and ubiquitination, a central region (aa 14-670) that contains 12 armadillo-like repeats and engages in protein-protein interaction, and a "flexible" C-terminus that also undergoes phosphorylation. beta -Catenin is widely, if not ubiquitously expressed, being found in endothelial cells, NKT cells, cortical neurons, neural stem cells, osteoblasts, fibroblasts, skeletal muscle cells, and Tregs. beta -Catenin is perhaps best known as a facilitator for Wnt signaling. In this context, and in the absence of Wnt signaling, non-nuclear or E-Cadherin-associated beta -Catenin is continually synthesized and degraded via a phosphorylation-dependent program. Upon Wnt engagement of its LPR:Fzd receptor complex, phosphorylation stops, allowing for an increase in it half-life and translocation into the nucleus. Here, it binds to TCF, generating a gene-activating transcriptional complex. Notably, Wnt signaling has been described as being beta -Catenin and Ca++/non-beta -Catenin based. It now appears they work in tandem, with Ca++ mobilization facilitating the translocation of beta -Catenin across the nuclear membrane. Human and mouse full-length beta -Catenin shows 99% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta ‑Catenin PE‑conjugated Monoclonal Antibody (Catalog # IC13292P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Intracellular Molecules. |
